PTEN (Phospho-Ser370) AntibodyPDF
Catalog Number:
E011062-2Amount:
100μg/100μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PTEN around the phosphorylation site of serine 370 (D-V-SP-D-N).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
PTEN (phospho-Ser370) antibody detects endogenous levels of PTEN only when phosphorylated at serine 370.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100Swiss-Prot No.:
P60484References:
Al-Khouri AM, et al. (2005). J Biol Chem.280 (42):35195-35202.Miller SJ, et al. (2002). FEBS Lett. 528(1-3): 145-153.Torres J, et al. (2001). J Biol Chem.276 (2): 993-998.Vazquez F, et al. (2000). Mol Cell Biol.20 (14): 5010-5018.P-Peptide - +Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PTEN (phospho-Ser370) antibody (E011062).
Western blot analysis of extracts from HeLa cells using PTEN (phospho-Ser370) antibody (E011062).
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
enogene的PTEN（Phospho-Ser380）抗体PDF
目录编号：E011009-1
量：50微克/ 50微升
抗体形式：磷酸盐缓冲盐水（不含Mg2 +和Ca2 +），pH 7.4、150mM NaCl，0.02％叠氮化钠和50％甘油的兔IgG。
储存/稳定性：-20℃/ 1年保存
免疫原产生抗人丝氨酸380（RY-SP-DT）磷酸化位点周围人PTEN合成的磷酸肽的抗血清。
纯化：使用表位特异性磷酸肽通过亲和层析从兔抗血清中亲和纯化抗体。使用对应于磷酸化位点的非磷酸肽，通过色谱法除去针对非磷酸肽的抗体。
特异性/敏感性：PTEN（phospho-Ser380）抗体仅在丝氨酸380磷酸化时才检测PTEN的内源性水平。
反应性：人，小鼠，大鼠
应用范围：IHC：1：50〜1：100
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