RFP-rProtein APDF

Catalog Number:
E1RA1-020
Amount:
200μg/200μl
Background:
The unique, high quality recombinant red fluorescence Protein (RFP)-rProtein A are produced in E. coli, and purified using multiple chromatographic steps (not affinity purified with human IgG). RFP-rProtein A bind to most mammalian immunoglobulins in their Fc binding domains and therefore are ideal for immunofluorescence staining. The genetically engineered protein was expressed and purified from transformed E. coli containing only the IgG binding regions and have 6X His-tag on their N-terminus. Other domains such as cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from all these proteins to ensure the maximum specific IgG binding and maximal fluorescence.
Form of Antibody:
Liquid. The product is 1 mg/ml in 10 mM Phosphate buffered saline, pH 7.4 containing, 10% glycerol and 0.1% sodium azide as preservative.
Source:
Recombinant expressed in E. coli
Purity:
>98% by SDS-PAGE and HPLC
Endotoxin:
< 0.05="">
IgG Binding:
>95%
Bioburden:
No organisms detected
Specificity/Sensitivity:
Under optimal conditions, 1 mg RFP-rProtein A will bind approximately 5 mg IgG. Optimal binding of RFP-rProtein A to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
Applications:
The protein is suitable as control reagent for RFP expression studies or as labeling reagent. Applications include: Flow cytometers, fluorescence microscopy, and microinjection, antibody purification, etc. at 1:100 dilution. Maximum absorption wavelength at 557 nm and emission wavelength at 585 nm.
Storage/Stability:
Store at -20°C. -80°C for long-term storage. Avoid exposure to light. Avoid repeated freezing and thawing.
Immunofluorescence staining of methanol-fixed HeLa cells using Synaptotagmin (Ab-309) Antibody (E021292) (Left) and PLC-γ1 (phospho-Tyr771) Antibody (E011523) (Right) consequently detected with RFP-rProtein A (Red).
Price:
160$