Catalog Number:
E011001-2Amount:
100μg/100μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human c-Jun around the phosphorylation site of serine 63 (L-T-SP-P-D).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
c-Jun (phospho-Ser63) antibody detects endogenous levels of c-Jun only when phosphorylated at serine 63.Reactivity:
Human, MouseApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100Swiss-Prot No.:
P05412References:
Wei W, et al. (2005). Cancer Cell. 8(1): 25-33.Sevilla A, et al. (2004). Oncogene.23 (55): 8950-8958.Li L, et al. (2004). J Biol Chem.279 (6): 4058-4065.Hurd C, et al. (2002). Oncogene.21 (14): 2154-2160.P-Peptide - +Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (phospho- ser63) antibody (E011001).
UV - +Western blot analysis of extracts from NIH/3T3 cells using c-Jun (phospho-Ser63) antibody (E011001)
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cellsenogene的PTEN(Phospho-Ser380)抗体PDF
目录编号:E011009-1
量:50微克/ 50微升
抗体形式:磷酸盐缓冲盐水(不含Mg2 +和Ca2 +),pH 7.4、150mM NaCl,0.02%叠氮化钠和50%甘油的兔IgG。
储存/稳定性:-20℃/ 1年保存
免疫原产生抗人丝氨酸380(RY-SP-DT)磷酸化位点周围人PTEN合成的磷酸肽的抗血清。
纯化:使用表位特异性磷酸肽通过亲和层析从兔抗血清中亲和纯化抗体。使用对应于磷酸化位点的非磷酸肽,通过色谱法除去针对非磷酸肽的抗体。
特异性/敏感性:PTEN(phospho-Ser380)抗体仅在丝氨酸380磷酸化时才检测PTEN的内源性水平。
反应性:人,小鼠,大鼠
应用范围:IHC:1:50〜1:100