PDK1 (Phospho-Ser241) AntibodyPDF
Catalog Number:
E011005-2Amount:
100μg/100μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241 (A-N-SP-F-V).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
PDK1 (phospho-Ser241) antibody detects endogenous levels of PDK1 only when phosphorylated at serine 241.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200Swiss-Prot No.:
O15530References:
Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63Chen H, et al. (2001) Biochemistry; 40(39): 11851-9Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11P-Peptide - +Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PDK1 (phospho-Ser241) antibody (E011005).
Western blot analysis of extracts from MDA-MB-435 cells using PDK1 (phospho-Ser241) antibody (E011005).
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
enogene的PTEN（Phospho-Ser380）抗体PDF
目录编号：E011009-1
量：50微克/ 50微升
抗体形式：磷酸盐缓冲盐水（不含Mg2 +和Ca2 +），pH 7.4、150mM NaCl，0.02％叠氮化钠和50％甘油的兔IgG。
储存/稳定性：-20℃/ 1年保存
免疫原产生抗人丝氨酸380（RY-SP-DT）磷酸化位点周围人PTEN合成的磷酸肽的抗血清。
纯化：使用表位特异性磷酸肽通过亲和层析从兔抗血清中亲和纯化抗体。使用对应于磷酸化位点的非磷酸肽，通过色谱法除去针对非磷酸肽的抗体。
特异性/敏感性：PTEN（phospho-Ser380）抗体仅在丝氨酸380磷酸化时才检测PTEN的内源性水平。
反应性：人，小鼠，大鼠
应用范围：IHC：1：50〜1：100
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