Catalog Number:
E011013-1Amount:
50μg/50μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human NF-κB around the phosphorylation site of serine 468 (L-A-SP-V-D).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
NF-κB p65 (phospho-Ser468) antibody detects endogenous levels of NF-κB p65 only when phosphorylated at serine 468.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IF:1:100~1:200Swiss-Prot No.:
Q04206References:
Buss H, et al. (2004) J Biol Chem.Schmitz ML, et al. (2004) Blood.Western blot analysis of extracts using NF-κB p65 (phospho-Ser468) antibody (E011013).
Immunofluorescence staining of methanol-fixed HeLa cellsusing NF-κB p65 (phospho-Ser468) antibody (E011013, Red).
Price:
145$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cellsenogene的PTEN(Phospho-Ser380)抗体PDF
目录编号:E011009-1
量:50微克/ 50微升
抗体形式:磷酸盐缓冲盐水(不含Mg2 +和Ca2 +),pH 7.4、150mM NaCl,0.02%叠氮化钠和50%甘油的兔IgG。
储存/稳定性:-20℃/ 1年保存
免疫原产生抗人丝氨酸380(RY-SP-DT)磷酸化位点周围人PTEN合成的磷酸肽的抗血清。
纯化:使用表位特异性磷酸肽通过亲和层析从兔抗血清中亲和纯化抗体。使用对应于磷酸化位点的非磷酸肽,通过色谱法除去针对非磷酸肽的抗体。
特异性/敏感性:PTEN(phospho-Ser380)抗体仅在丝氨酸380磷酸化时才检测PTEN的内源性水平。
反应性:人,小鼠,大鼠
应用范围:IHC:1:50〜1:100