Catalog Number:
E0L3012-2Amount:
1000ulForm of Antibody:
HRP-labeled Goat IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was developed in goat using the rabbit IgG as the immunogen.Purification:
The antibody is obtained by affinity purified technique, which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. Goat anti-rabbit IgG is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK.Specificity/Sensitivity:
The antibody conjugate is specific for rabbit IgG. No cross-reactivity was observed with rabbit IgA, IgM proteins by ELISA.Applications:
ELISA : 1:5000~1:20000, WB : 1:5000~1:10000, IHC 1:1000~5000References:
Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).Price:
100$enogene的PTEN(Phospho-Ser380)抗体PDF
目录编号:E011009-1
量:50微克/ 50微升
抗体形式:磷酸盐缓冲盐水(不含Mg2 +和Ca2 +),pH 7.4、150mM NaCl,0.02%叠氮化钠和50%甘油的兔IgG。
储存/稳定性:-20℃/ 1年保存
免疫原产生抗人丝氨酸380(RY-SP-DT)磷酸化位点周围人PTEN合成的磷酸肽的抗血清。
纯化:使用表位特异性磷酸肽通过亲和层析从兔抗血清中亲和纯化抗体。使用对应于磷酸化位点的非磷酸肽,通过色谱法除去针对非磷酸肽的抗体。
特异性/敏感性:PTEN(phospho-Ser380)抗体仅在丝氨酸380磷酸化时才检测PTEN的内源性水平。
反应性:人,小鼠,大鼠
应用范围:IHC:1:50〜1:100